Journal: Cancer Medicine

Article Title: Overexpression of claspin promotes docetaxel resistance and is associated with prostate‐specific antigen recurrence in prostate cancer

doi: 10.1002/cam4.4113

Figure Lengend Snippet: Effect of claspin inhibition on cell proliferation. (A) Western blot analysis of claspin in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs. (B) qRT‐PCR analysis of CLSPN in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs. (C) Effects of claspin knockdown on DU145 and PC3 cell proliferation. An MTT assay assessed cell proliferation at 1, 2, and 4 days after seeding on 96‐well plates. Bars and error bars indicate the mean and S.D., respectively

Article Snippet: DU145, PC3, and LNCaP were purchased from the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Inhibition, Western Blot, Transfection, Negative Control, Quantitative RT-PCR, Knockdown, MTT Assay

Journal: Cancer Medicine

Article Title: Overexpression of claspin promotes docetaxel resistance and is associated with prostate‐specific antigen recurrence in prostate cancer

doi: 10.1002/cam4.4113

Figure Lengend Snippet: Association of claspin inhibition with DNA damage and resistance to docetaxel (DTX). (A) Dose‐dependent effect of DTX on the viability of DU145 and PC3 cells analyzed by MTT assay. Bars and error bars indicate the mean and S.D., respectively. (B) Western blot analysis of γ‐H2AX expressions in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs after 24 h of treatment with DTX. (C) Western blot analysis of cleaved PARP expressions in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs after 24 h of treatment with DTX

Article Snippet: DU145, PC3, and LNCaP were purchased from the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Inhibition, MTT Assay, Western Blot, Transfection, Negative Control

Journal: Cancer Medicine

Article Title: Overexpression of claspin promotes docetaxel resistance and is associated with prostate‐specific antigen recurrence in prostate cancer

doi: 10.1002/cam4.4113

Figure Lengend Snippet: Effect of claspin inhibition on Akt, Erk, and ATR‐CHK1 pathways. (A) Western blot analysis of claspin, Akt, p‐Akt, Erk1/2, and p‐Erk1/2 in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs. (B) Western blot analysis of claspin, CHK1, and p‐CHK1 in DU145 and PC3 cells transfected with CLSPN or negative control siRNAs

Article Snippet: DU145, PC3, and LNCaP were purchased from the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Inhibition, Western Blot, Transfection, Negative Control

Journal: Cancer Medicine

Article Title: Overexpression of claspin promotes docetaxel resistance and is associated with prostate‐specific antigen recurrence in prostate cancer

doi: 10.1002/cam4.4113

Figure Lengend Snippet: Effect of claspin inhibition on the docetaxel (DTX)‐resistant DU145 (DU145‐DR) cell line. (A) Western blot analysis of claspin, Akt, p‐Akt, Erk1/2, and p‐Erk1/2 in DU145 and DU145‐DR cells transfected with CLSPN or negative control siRNAs. (B) Western blot analysis of claspin, CHK1, and p‐CHK1 in DU145‐DR cells transfected with CLSPN or negative control siRNAs. (C) Effects of claspin knockdown on DU145‐DR cell proliferation that was assessed by an MTT assay. (D) Dose‐dependent effect of DTX on the viability of DU145‐DR cells analyzed by MTT assay. Bars and error bars indicate the mean and S.D., respectively. (E) Western blot analysis of claspin, Akt, p‐Akt, ERK1/2, p‐ERK1/2, CHK1, and p‐CHK1 in DU145‐DR cells transfected with CLSPN or negative control siRNAs

Article Snippet: DU145, PC3, and LNCaP were purchased from the Japanese Collection of Research Bioresources Cell Bank.

Techniques: Inhibition, Western Blot, Transfection, Negative Control, Knockdown, MTT Assay